Accurate and rapid detection of Fasciola hepatica copro‐DNA in sheep using loop‐mediated isothermal amplification (LAMP) technique
نویسندگان
چکیده
منابع مشابه
تشخیص و افتراق سریع فاسیولا هپاتیکا و فاسیولا ژیگانتیکا با استفاده از روش loop-mediated Isothermal Amplification
Background and purpose: Fasciola hepatica and Fasciola gigantica are common liver flukes which affect both human and livestock worldwide. In this study we evaluated the loop-mediated isothermal amplification (LAMP) assay for detection and discrimination of Fasciola species. Materials and methods: Fifty adults of Fasciola worms were isolated from sheep and cattle liver form abattoirs in Mazand...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملMolecular differentiation of sheep and cattle isolates of Fasciola hepatica using RAPD-PCR
Understanding genetic structure and status of genetic variation of Fasciola hepatica isolates from different hosts, has important implications on epidemiology and effective control of fasciolosis. Random amplified polymorphic DNA (RAPD-PCR) was used to study the genetic variation of F. hepatica in sheep and cattle. DNA was extracted from adult helminthes removed from livers of each infected ani...
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ژورنال
عنوان ژورنال: Veterinary Medicine and Science
سال: 2021
ISSN: 2053-1095,2053-1095
DOI: 10.1002/vms3.455